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Dry Film Photoresist-based Electrochemical Microfluidic Biosensor Platform: Device Fabrication, On-chip Assay Preparation, and System Operation

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Recent Advances in Digital Biosensing Technology.

Kathrine Curtin¹, Bethany J Fike², Brandi Binkley²

¹Department of Mechanical and Aerospace Engineering, West Virginia University, Morgantown, WV 26506, USA.

|September 23, 2022

Summary

Digital biosensing assays offer superior single-molecule detection and absolute quantification. This technology significantly reduces sample volumes and reagent use, enhancing sensitivity and throughput for detecting proteins and nucleic acids.

Keywords:

biosensor digital ELISA digital PCR digital biosensing microfluidics

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Area of Science:

Biotechnology
Analytical Chemistry
Molecular Diagnostics

Background:

Conventional biosensing assays face limitations in sensitivity and require large sample volumes.
Digital biosensing offers a paradigm shift with single-molecule detection capabilities.
Current digital biosensing systems significantly reduce reagent consumption and reaction times.

Purpose of the Study:

To review current technologies for reaction compartmentalization in digital biosensing.
To highlight applications of digital biosensing in detecting proteins and nucleic acids.
To analyze challenges and future opportunities in integrated digital biosensing systems.

Main Methods:

Review of existing literature on digital biosensing technologies.
Analysis of compartmentalization techniques for digital assays.
Examination of applications in protein and nucleic acid detection.

Main Results:

Digital biosensing enables absolute quantification and single-molecule detection.
Reduced sample volumes (fL-nL) lead to lower reagent consumption and faster assays.
High sensitivity and throughput are key advantages over conventional methods.

Conclusions:

Digital biosensing represents a significant advancement in biomolecular detection.
Further development of integrated systems promises broader applications.
Addressing current challenges will unlock future opportunities in diagnostics and research.