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Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

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Flow-Through Acetylcholinesterase Sensor with Replaceable Enzyme Reactor.

Alexey Ivanov¹, Dmitry Stoikov¹, Insiya Shafigullina¹

¹A.M. Butlerov' Chemistry Institute, Kazan Federal University, 18 Kremlevskaya Street, 420008 Kazan, Russia.

|September 23, 2022

Summary

This study presents a 3D-printed biosensor for rapid enzyme inhibitor detection. The developed acetylcholinesterase biosensor offers sensitive and reliable analysis of environmental and biomedical contaminants.

Keywords:

acetylcholinesterase sensor electropolymerization flow-through analysis inhibitor determination pillar[5]arene

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Published on: September 23, 2013

Area of Science:

Biomedical Engineering
Environmental Science
Analytical Chemistry

Background:

Enzyme inhibitors are crucial in environmental monitoring and biomedicine due to their toxicity.
Accurate assessment of enzyme inhibitors in various media is essential.

Purpose of the Study:

To develop a 3D-printed flow-through biosensor for the rapid determination of enzyme inhibitors.
To immobilize acetylcholinesterase (AChE) and detect thiocholine amperometrically.

Main Methods:

Fabrication of a 3D-printed poly(lactic acid) flow-through reactor.
Immobilization of electric eel acetylcholinesterase (AChE).
Amperometric detection of thiocholine using a modified screen-printed carbon electrode.

Main Results:

The biosensor successfully detected reversible (donepezil, berberine) and irreversible (carbofuran) inhibitors.
Optimal conditions allowed detection of donepezil (1.0 nM-1.0 μM), berberine (1.0 μM-1.0 mM), and carbofuran (10 nM-0.1 μM).
The biosensor demonstrated reliable performance in spiked artificial urine and peanut samples.

Conclusions:

The 3D-printed AChE biosensor provides a cost-effective, sensitive, and reliable method for enzyme inhibitor determination.
Its ease of use and satisfactory analytical characteristics suggest potential for point-of-care applications outside laboratories.