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A Ratiometric Organic Fluorescent Nanogel Thermometer for Highly Sensitive Temperature Sensing
Chao Wang1, Xianhao Zhao1, Kaiyu Wu1
1Key Laboratory of Functional Polymer Materials of Ministry of Education, State Key Laboratory of Medicinal Chemical Biology, Institute of Polymer Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China.
Biosensors
|September 23, 2022
Summary
Researchers developed a novel fluorescent nanogel thermometer for precise biological temperature sensing. This advanced nanothermometer offers high sensitivity for determining bactericidal temperatures and predicting photothermal antibacterial effects.
Area of Science:
- Biomedical Engineering
- Materials Science
- Nanotechnology
Background:
- Accurate temperature sensing is crucial for understanding biological processes.
- Developing sensitive organic fluorescent nanothermometers for biological applications presents significant challenges.
Purpose of the Study:
- To develop a ratiometric fluorescent nanogel thermometer for sensitive temperature detection.
- To investigate its application in determining bactericidal temperatures and predicting photothermal effects.
Main Methods:
- Synthesized a nanogel incorporating a dual-emission aggregation-induced emission luminogen and a thermoresponsive polymer.
- Utilized the nanogel's reversible spectral shift during temperature-induced phase transition for ratiometric sensing.
- Established a correlation between emission intensity ratio and temperature in aqueous and simulated physiological environments.
Main Results:
- The nanogel demonstrated reversible and sensitive spectral changes (red to blue emission) in response to temperature-induced hydrophobicity changes.
- Achieved high relative thermal sensitivities of 128.42% °C⁻¹ in water and 68.39% °C⁻¹ in simulated physiological conditions.
- Successfully applied the nanogel to indicate bactericidal temperatures visually and ratiometrically.
Conclusions:
- The developed ratiometric fluorescent nanogel thermometer exhibits excellent sensitivity and responsiveness for biological temperature sensing.
- This nanothermometer shows significant potential for applications in rapid prediction of photothermal antibacterial efficacy and other temperature-dependent biological events.
