Histostar-Functionalized Covalent Organic Framework for Electrochemical Detection of Exosomes

Covalent organic frameworks (COFs) are gaining growing interest owing to their various structures and versatility. Since their specific physical-chemical characteristics endow them great usage potentiality in biosensing, we herein have synthesized spherical COFs with regular shape and good dispersion, which are further used for the design of a novel nanoprobe by modifying Histostar on the surface of the COFs. Moreover, we have applied a nanoprobe for the fabrication of an electrochemical biosensor to detect exosomes. Since Histostar is a special polymer, conjugated with many secondary antibodies (IgG), and HRP can increase the availability of HRP at the antigenic site, the biosensor can have a strong signal amplification ability. Meanwhile, since COFs with high porosity can be loaded with a huge amount of Histostar, the sensitivity of the biosensor can be further improved. With such a design, the proposed biosensor can achieve a low exosomes detection limit of 318 particles/µL, and a wide linear detection range from 10³ particles/µL to 10⁸ particles/µL. So, this work may offer a promising platform for the ultrasensitive detection of exosomes.