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Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

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Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Hundreds-Dollar-Level Multiplex Integrated RT-qPCR Quantitative System for Field Detection.

Zhihao Lan^1,2, Yu Guo³, Kangning Wang¹

¹Institute of Biological and Medical Engineering, Guangdong Academy of Sciences, Guangzhou 510075, China.

|September 23, 2022

Summary

A new, low-cost portable real-time PCR device enables rapid, on-site disease detection, crucial for developing nations. This innovation accelerates diagnosis, bypassing the need for central laboratories and improving outbreak control.

Keywords:

ASF COVID-19 fluorescence detection low cost on-site testing real-time PCR

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Last Updated: Aug 28, 2025

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

Author Spotlight: Advancements in Multiplex Detection of Respiratory Viruses

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Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Two-Step Reverse Transcription Droplet Digital PCR Protocols for SARS-CoV-2 Detection and Quantification

Published on: March 31, 2021

Area of Science:

Molecular Biology
Medical Diagnostics
Biotechnology

Background:

Real-time PCR is vital for COVID-19 diagnosis but inaccessible in many developing nations.
Lack of portable, affordable PCR equipment hinders timely on-site disease detection and outbreak control.
Existing diagnostic methods face delays due to sample transportation to central laboratories.

Purpose of the Study:

To develop a low-cost, portable, multiplexed real-time fluorescence detection apparatus for on-site disease testing.
To address the need for accessible molecular diagnostic tools in resource-limited settings.
To enable rapid and sensitive on-site testing for diverse diseases.

Main Methods:

Development of the HDLRT-qPCR apparatus, a novel, low-cost, multiplexed real-time fluorescence detection instrument.
Testing the apparatus for gradient amplification and melting curve analysis.
Validation of the instrument's performance against commercially available equipment.

Main Results:

The HDLRT-qPCR apparatus achieved gradient amplification and melting curves comparable to commercial equipment.
Testing outcomes demonstrated good consistency and reliability in detecting target genes.
The entire instrument cost is under USD 760, making it highly affordable.

Conclusions:

The developed HDLRT-qPCR instrument provides a reliable, inexpensive solution for on-site molecular diagnostics.
This portable PCR device significantly accelerates testing speed, delivering results within 40 minutes.
The apparatus is suitable for diverse disease investigations in developing nations, enhancing public health response.