Recent Progresses in Development of Biosensors for Thrombin Detection

Area of Science:

• Biochemistry
• Biotechnology
• Analytical Chemistry

Background:

• Thrombin is a crucial serine protease in hemostasis and blood coagulation.
• It plays a vital role in converting fibrinogen to fibrin and activating platelets during vascular injury.
• Accurate thrombin detection is essential for biological analysis and clinical diagnostics.

Purpose of the Study:

• To review and update the literature on biosensors designed for thrombin detection.
• To compare different biosensor designs based on their transducers, compositions, performance, benefits, and limitations.

Main Methods:

• Literature review of biosensors for thrombin detection.
• Categorization of biosensors based on transducer types and design principles.
• Comparative analysis of sensor performance, advantages, and disadvantages.

Main Results:

• Various biosensor platforms have been developed for thrombin measurement.
• Aptasensors, utilizing DNA or RNA aptamers, demonstrate high sensitivity and specificity.
• Different biosensor designs offer varying levels of performance, benefits, and limitations.

Conclusions:

• Biosensors provide effective tools for selective and specific thrombin detection.
• Aptasensors represent a promising approach for sensitive thrombin quantification.
• Further research and development can optimize biosensor performance for clinical applications.