Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Telomeres and Telomerase02:41

Telomeres and Telomerase

23.8K
In eukaryotic DNA replication, a single-stranded DNA fragment remains at the end of a chromosome after the removal of the final primer. This section of DNA cannot be replicated in the same manner as the rest of the strand because there is no 3’ end to which the newly synthesized DNA can attach. This non-replicated fragment results in gradual loss of the chromosomal DNA during each cell duplication. Additionally, it can induce a DNA damage response by enzymes that recognize single-stranded...
23.8K
Replication in Eukaryotes01:29

Replication in Eukaryotes

14.2K
In eukaryotic cells, DNA replication is highly conserved and tightly regulated. Multiple linear chromosomes must be duplicated with high fidelity before cell division, so there are many proteins that fulfill specialized roles in the replication process. Replication occurs in three phases: initiation, elongation, and termination, and ends with two complete sets of chromosomes in the nucleus.
Many Proteins Orchestrate Replication at the Origin
Eukaryotic replication follows many of the same...
14.2K
Replicative Cell Senescence02:15

Replicative Cell Senescence

3.7K
Replicative cell senescence is a property of cells that allows them to divide a finite number of times throughout the organism's lifespan while preventing excessive proliferation. Replicative senescence is associated with the gradual loss of the telomere — short, repetitive DNA sequences found at the end of the chromosomes. Telomeres are bound by a group of proteins to form a protective cap on the ends of chromosomes. Embryonic stem cells express telomerase — an enzyme that adds...
3.7K
Chromosome Structure02:40

Chromosome Structure

23.2K
A functional eukaryotic chromosome must contain three elements: a centromere, telomeres, and numerous origins of replication.
The centromere is a DNA sequence that links sister chromatids. This is also where kinetochores, protein complexes to which spindle microtubules attach, are constructed after the chromosome is replicated. The kinetochores allow the spindle microtubules to move the chromosomes within the cell during cell division.
Telomeres consist of non-coding repetitive nucleotide...
23.2K
Histone Variants at the Centromere02:30

Histone Variants at the Centromere

4.5K
Histone variants are the histone proteins with structural and sequence variations. These variants may be regarded as “mutant” forms that replace their canonical histone counterparts in the nucleosomes. Specific post-translational modifications on the histone variants enable further chromatin complexity and regulate tissue-specific gene expression. The most common histone variants are from histone H2A, H2B, and linker histone H1 families. However, several variants of histone H3...
4.5K
RNA Structure01:19

RNA Structure

5.1K
The basic structure of RNA consists of a string of ribonucleotides attached by phosphodiester bonds. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA) involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three...
5.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

La protein binding to telomerase RNA supports an evolutionary relationship between plant and ciliate telomerase pathways.

Nucleic acids research·2026
Same author

Human ARMC6 binds in vitro to both cancer genes and telomeric RNA, favoring G-quadruplex structure recognition.

Biochimica et biophysica acta. Gene regulatory mechanisms·2024
Same author

Characterisation of the Arabidopsis thaliana telomerase TERT-TR complex.

Plant molecular biology·2024
Same author

TeloBase: a community-curated database of telomere sequences across the tree of life.

Nucleic acids research·2023
Same author

Telomerase RNA gene paralogs in plants - the usual pathway to unusual telomeres.

The New phytologist·2023
Same author

Identification of the Sequence and the Length of Telomere DNA.

Methods in molecular biology (Clifton, N.J.)·2023

Related Experiment Video

Updated: Aug 28, 2025

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

827

Telomeres and Their Neighbors.

Leon P Jenner1, Vratislav Peska1, Jana Fulnečková1

  • 1Institute of Biophysics of the Czech Academy of Sciences, CZ-61265 Brno, Czech Republic.

Genes
|September 23, 2022
PubMed
Summary

This review explores telomeres and satellite DNA in non-model organisms, offering accessible methods for identifying these crucial genetic elements. It aims to inspire new research into their biological functions beyond traditional models.

Keywords:
FISHNGSTRAPeukaryotic tree of lifeinterstitial telomere sequencesretroelementssatellitesubtelomere structuretelomerase RNAtelomere evolution

More Related Videos

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.6K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.1K

Related Experiment Videos

Last Updated: Aug 28, 2025

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions
11:21

Author Spotlight: Advanced Single-Molecule Techniques for Investigating Telomeric Protein-DNA Interactions

Published on: August 30, 2024

827
Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence
12:08

Telomere Length and Telomerase Activity; A Yin and Yang of Cell Senescence

Published on: May 22, 2013

46.6K
Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization
11:29

Modified Terminal Restriction Fragment Analysis for Quantifying Telomere Length Using In-gel Hybridization

Published on: July 10, 2017

13.1K

Area of Science:

  • Genetics
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Telomeres, composed of satellite DNA repeats, cap chromosome ends in eukaryotes.
  • Satellite DNA sequences serve as karyotyping markers but their broader biological roles remain enigmatic.
  • Research has extensively studied repetitive DNA in model organisms, yet gaps exist for non-model species.

Purpose of the Study:

  • To inspire and guide new research on telomeres and satellite DNA, focusing on non-model organisms.
  • To present accessible experimental and in silico methods for identifying telomere and satellite repeats.
  • To broaden the understanding of repetitive DNA functions beyond traditional human and yeast models.

Main Methods:

  • Review of established and novel techniques for identifying telomere and satellite DNA repeats.
  • Inclusion of published and unpublished data examples to illustrate experimental principles.
  • Guidance on performing and analyzing experiments, including common pitfalls.

Main Results:

  • Detailed introduction to current knowledge of telomere and satellite systems.
  • Examples from plants and insects highlight recent developments and underexplored research areas.
  • Emphasis on accessible methods suitable for non-model organism research.

Conclusions:

  • Further research is needed to fully elucidate the biological functions of satellite DNA sequences, especially in non-model organisms.
  • Accessible methodologies can facilitate broader investigation into telomeres and satellites.
  • Expanding research beyond model organisms offers new insights into species evolution and genetic diversity.