Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The H-2Kkml mutation: nucleotide sequence and comparative analysis.

J M Martinko, J C Solheim, J Geliebter

    Molecular Immunology
    |February 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Interacting Genetic Lesions of Melanoma in the Tumor Microenvironment: Defining a Viable Therapy.

    Advances in experimental medicine and biology·2021
    Same author

    Interlinking of hypoxia and estrogen in thyroid cancer progression.

    Current medicinal chemistry·2013
    Same author

    Analysis of major histocompatibility complex class I folding: novel insights into intermediate forms.

    Tissue antigens·2012
    Same author

    Comparative analysis of the impact of a free cysteine in tapasin on the maturation and surface expression of murine MHC class I allotypes.

    International journal of immunogenetics·2009
    Same author

    Spleen but not tumor infiltration by dendritic and T cells is increased by intravenous adenovirus-Flt3 ligand injection.

    Cancer gene therapy·2007
    Same author

    Pilot study using SELDI-TOF-MS based proteomic profile for the identification of diagnostic biomarkers of thyroid proliferative diseases.

    Journal of proteome research·2006
    Same journal

    Neutrophil elastase-SERPINB1 axis in epithelial responses: Cell death, inflammation and SERPINB1 dynamics.

    Molecular immunology·2026
    Same journal

    Phillyrin alleviates pulmonary fibrosis via modulation of the TGF-β1/Smad and Nrf2/HO-1 signaling pathways.

    Molecular immunology·2026
    Same journal

    m6A modification of LINC00458 enhances HMOX1 stability via ELAVL1 recruitment to promote ferroptosis and aggravate asthma.

    Molecular immunology·2026
    Same journal

    Overexpression of Hes1 inhibits cigarette smoke-induced mitochondrial apoptosis in AT2 cells by activating the Pgc-1α/Tfam signaling pathway.

    Molecular immunology·2026
    Same journal

    Progesterone promotes favorable pregnancy outcomes in recurrent spontaneous, abortion by attenuating NK Cell overactivation and upregulating the cAMP/PKA/CREB signaling axis.

    Molecular immunology·2026
    Same journal

    Oleanolic acid alleviates hepatic fibrosis by inhibiting liver macrophage recruitment and polarization.

    Molecular immunology·2026
    See all related articles

    Mutation analysis of the H-2Kkml mutant revealed a key amino acid change at position 152, altering its biological activity. This specific mutation suggests a gene conversion event in major histocompatibility complex (MHC) class I variants.

    Area of Science:

    • Immunogenetics
    • Molecular Biology
    • Genomics

    Background:

    • Class I Major Histocompatibility Complex (MHC) molecules are crucial for immune responses.
    • Mutations in MHC genes can lead to altered immune function and disease susceptibility.
    • The H-2Kkml is a murine mutant with a known defect in immune recognition.

    Purpose of the Study:

    • To pinpoint the exact mutation site in the H-2Kkml mutant.
    • To understand the molecular basis of altered biological activity in H-2Kkml.
    • To investigate the origin of the observed mutation in the H-2Kkml gene.

    Main Methods:

    • Nucleotide sequence analysis of messenger RNA (mRNA) from H-2Kkml.
    • Comparative sequence analysis of MHC class I variants and pseudogenes.

    Related Experiment Videos

  • Amino acid sequence determination based on nucleotide sequences.

    • Main Results:

    • The mutation in H-2Kkml was localized to the codon for amino acid position 152.
    • A specific nucleotide alteration (A to C) changed Aspartic acid (GAT) to Alanine (GCT) at position 152.
    • Similar alterations at position 152 are found in other MHC class I variants, affecting biological activity.
    • The H-2k haplotype pseudogene (pH-2III) also possesses a GCT codon at position 152.

    Conclusions:

    • The mutation at amino acid 152 in H-2Kkml is responsible for its altered biological activity.
    • The presence of the GCT codon in both H-2Kkml and the H-2k pseudogene suggests a gene conversion event.
    • This finding provides insights into the mechanisms of MHC gene evolution and variation.