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Rose Bengal-Modified Upconverting Nanoparticles: Synthesis, Characterization, and Biological Evaluation
Mykhailo Nahorniak1, Ognen Pop-Georgievski1, Nadiia Velychkivska1
1Institute of Macromolecular Chemistry, Czech Academy of Sciences, Heyrovského nám. 2, 162 06 Prague, Czech Republic.
Life (Basel, Switzerland)
|September 23, 2022
Summary
Rose Bengal-conjugated upconverting nanoparticles show promise for cancer photodynamic therapy. These nanoparticles generate reactive oxygen species upon irradiation, selectively killing cancer cells while sparing healthy cells.
Area of Science:
- Nanotechnology
- Materials Science
- Biomedical Engineering
Background:
- Upconverting nanoparticles (UCNPs) offer unique optical properties for biomedical applications.
- Lanthanide-doped NaYF4:Yb3+,Er3+ nanoparticles were synthesized for their efficient upconversion luminescence.
- Modification with poly(ethylene glycol) (PEG) and Rose Bengal (RB) photosensitizer enhances their functionality.
Purpose of the Study:
- To synthesize and characterize PEG-modified UCNPs conjugated with Rose Bengal.
- To investigate the singlet oxygen generation and photodynamic efficacy of these UCNPs.
- To evaluate the cytotoxicity of the UCNPs on rat mesenchymal stem cells (rMSCs) and rat glioma cells (C6).
Main Methods:
- High-temperature coprecipitation for UCNP synthesis.
- Characterization using TEM, DLS, TGA, FTIR, XPS, and upconversion luminescence spectroscopy.
- Evaluation of singlet oxygen generation and in vitro cytotoxicity assays.
Main Results:
- Synthesized 26 nm UCNPs were successfully modified with PEG and RB.
- RB-conjugated UCNPs demonstrated efficient singlet oxygen generation under 980 nm excitation.
- RB-conjugated UCNPs showed selective toxicity towards C6 glioma cells upon irradiation, with minimal impact on rMSCs.
Conclusions:
- PEG-modified UCNPs conjugated with RB are effective in generating reactive oxygen species for photodynamic therapy.
- These nanoparticles exhibit promising selective cytotoxicity against cancer cells, suggesting potential for targeted cancer treatment.
- The findings support the application of RB-conjugated PEG-UCNPs in photodynamic cancer therapy.
