Electrochemical Detection of Ascorbic Acid in Finger-Actuated Microfluidic Chip
Xing Liu1, Mi Li1, Jiahui Zheng1
1Laboratory of Biorheological Science and Technology, Ministry of Education, Bioengineering College, Chongqing University, Chongqing 400030, China.
Micromachines
|September 23, 2022
Summary
This study presents a low-cost, high-efficiency device for detecting ascorbic acid (AA). The portable system offers sensitive, rapid AA quantification with minimal sample volume, ideal for point-of-care testing.
Area of Science:
- Analytical Chemistry
- Biomedical Engineering
- Materials Science
Background:
- Traditional ascorbic acid (AA) analysis is complex, time-consuming, and requires expensive equipment.
- There is a need for accessible, efficient methods for AA quantification.
Purpose of the Study:
- To develop a low-cost, high-efficiency device for rapid ascorbic acid detection.
- To demonstrate the device's utility in point-of-care testing (POCT) applications.
Main Methods:
- Integration of a screen-printed three-electrode sensor with a microfluidic chip.
- Utilized a 3D-printed finger-actuated micropump for sample handling.
- Employed electrochemical detection for ascorbic acid quantification.
Main Results:
- Achieved on-chip detection sensitivity 2.48 times higher than off-chip methods.
- Required only microliter-scale sample volumes, significantly reducing sample requirements.
- Demonstrated >90% mixing efficiency within the microchannel via numerical simulation and experiments.
- Validated device accuracy and reliability using commercial tablets and beverages.
Conclusions:
- The developed device offers a sensitive, efficient, and user-friendly approach for ascorbic acid detection.
- Its low cost and portability make it suitable for point-of-care testing.
- The technology shows broad application prospects for rapid biochemical analysis.
