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A Bright NIR-II Fluorescence Probe for Vascular and Tumor Imaging
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Recent Progress on NIR Fluorescent Probes for Enzymes.

Jintao Zhao1, Tao Ma1, Bingbing Chang1

  • 1State Key Laboratory of Applied Organic Chemistry, College of Chemistry and Chemical Engineering, Lanzhou University, Lanzhou 730000, China.

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|September 23, 2022
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Summary

Enzyme activity is crucial for health, and abnormal levels indicate disease. Near-infrared (NIR) fluorescent probes offer advanced imaging for studying enzyme function and distribution in biological systems.

Keywords:
enzymefluorescent probenear-infrared

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Medical Imaging

Background:

  • Enzymes are key biomarkers for many diseases, and their dysregulation is linked to biological disorders.
  • Understanding enzyme activity, function, and distribution is fundamental for diagnosing and treating diseases.
  • Near-infrared (NIR) fluorescence imaging provides unique advantages for in vivo studies of biological processes.

Purpose of the Study:

  • To review design strategies for near-infrared (NIR) fluorescent probes used as enzyme sensors.
  • To summarize recent advancements in enzyme-activated NIR fluorescent probes and their imaging applications.
  • To discuss the future prospects and challenges in developing novel enzyme-activated NIR fluorescent probes.

Main Methods:

  • Literature review of NIR fluorescent probe design strategies for enzyme sensing.
  • Analysis of recent research on enzyme-activated NIR fluorescent probes.
  • Synthesis of information on the applications of these probes in biological imaging.

Main Results:

  • NIR fluorescent probes offer intuitive insights into enzyme studies within biological systems.
  • Various design strategies have been successfully employed to create effective enzyme sensors.
  • Recent enzyme-activated probes demonstrate significant potential for advanced biological imaging.

Conclusions:

  • Enzyme-activated NIR fluorescent probes are powerful tools for studying enzyme roles in vivo.
  • Continued development of these probes is essential for advancing our understanding of enzyme-related diseases.
  • Addressing current challenges will unlock new diagnostic and therapeutic possibilities.