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Chitosan Based MicroRNA Nanocarriers.

Hussein H Genedy1, Thierry Delair1, Alexandra Montembault1

  • 1Université de Lyon, CNRS, Université Claude Bernard Lyon 1, INSA Lyon, Université Jean Monnet, UMR 5223, Ingénierie des Matériaux Polymères, CEDEX, 69629 Villeurbanne, France.

Pharmaceuticals (Basel, Switzerland)
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Chitosan nanoparticles offer a promising solution for microRNA delivery, overcoming challenges in stability and targeting for disease treatment. This review details chitosan-based microRNA formulations over the past decade.

Keywords:
ChitosanmicroRNAnanoparticlepolymerpolyplexpolysaccharide

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Gene Therapy

Background:

  • MicroRNA (miRNA) vectorization shows therapeutic potential for diseases like cancer and infections.
  • In vivo miRNA delivery faces challenges in stability, targeting, specificity, and cellular uptake.
  • Polymeric nanoparticles, particularly chitosan, are promising for overcoming these delivery barriers.

Purpose of the Study:

  • To review chitosan-based microRNA formulations developed in the last 10 years.
  • To analyze differences in materials, formulation processes, and applications of these systems.
  • To identify factors contributing to the optimization of chitosan microRNA nanocarriers.

Main Methods:

  • Comprehensive literature review of chitosan microRNA formulations.
  • Analysis of material properties, formulation techniques, and therapeutic targets.
  • Evaluation of system performance and optimization strategies.

Main Results:

  • Chitosan's cationic nature facilitates complexation with anionic polymers for miRNA encapsulation.
  • Various chitosan-based formulations have been explored for diverse therapeutic applications.
  • Optimization of chitosan characteristics is crucial for effective miRNA delivery systems.

Conclusions:

  • Chitosan is a versatile biomaterial for developing effective microRNA nanocarriers.
  • Understanding formulation parameters is key to enhancing miRNA delivery efficiency and therapeutic outcomes.
  • Further research into optimized chitosan systems will unlock the full potential of miRNA-based therapies.