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In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
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Morphogenesis Dynamics in Leishmania Differentiation.

Ramu Dandugudumula1, Renana Fischer-Weinberger1, Dan Zilberstein1

  • 1Faculty of Biology, Technion-Israel Institute of Technology, Haifa 3200003, Israel.

Pathogens (Basel, Switzerland)
|September 23, 2022
PubMed
Summary
This summary is machine-generated.

Microtubule remodeling is not essential for Leishmania promastigote rounding into amastigotes. However, amastigote elongation into promastigotes requires microtubule rearrangement, highlighting distinct developmental pathways for this parasite.

Keywords:
differentiationimage streamingleishmaniamorphogenesissubpellicular microtubules

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Area of Science:

  • Cell Biology
  • Parasitology
  • Microbiology

Background:

  • Leishmania parasites, causative agents of leishmaniasis, exhibit distinct life cycle stages: extracellular promastigotes in sandflies and intracellular amastigotes in macrophages.
  • The parasite's cytoskeleton, crucial for morphology, comprises stable subpellicular microtubules (SPMTs).
  • Understanding developmental morphogenesis and the role of microtubule dynamics is key to parasite biology.

Purpose of the Study:

  • To investigate the kinetics of Leishmania developmental morphogenesis.
  • To determine the involvement of microtubule remodeling in parasite differentiation.
  • To elucidate the role of specific proteins in parasite shape changes.

Main Methods:

  • Utilized image-streaming technology to observe parasite differentiation in real-time.
  • Employed microtubule-stabilizing (taxol) and destabilizing (vinblastine) agents to assess their effects on parasite morphology.
  • Studied Leishmania mutants lacking specific protein kinase A (PKA) components.

Main Results:

  • Promastigote rounding into amastigotes occurred rapidly and was not dependent on microtubule remodeling.
  • Microtubule stabilization accelerated rounding but led to parasite death if not removed; destabilization had no effect on rounding.
  • Amastigote elongation into promastigotes involved a delay, sensitivity to microtubule destabilizers, and eventual microtubule rearrangement.
  • Mutants lacking PKA components showed defects in promastigote elongation but not amastigote rounding.

Conclusions:

  • Leishmania promastigote rounding into amastigotes is independent of microtubule remodeling.
  • Amastigote to promastigote morphogenesis requires significant microtubule rearrangement.
  • This study provides the first insights into microtubule dynamics during Leishmania development.