Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Hessian fly tolerance increases yield in elite durum wheat cultivars Svevo and Soft Svevo.

Communications biology·2026
Same author

Sorghum ferulate-5-hydroxylase overexpression enhances aphid proliferation and weakens brown midrib12-linked resistance.

Journal of experimental botany·2026
Same author

Insights Into Virus-Encoded RNA Silencing Suppressors Across Viral Families: A Focus on Viruses Infecting Solanaceae Crops.

Physiologia plantarum·2026
Same author

Flavonoids at the crossroads of plant defence: a multifunctional shield against insect pests.

Journal of experimental botany·2026
Same author

Breeding vegetables for whitefly resistance: past, present, and future in the AI era.

Frontiers in plant science·2026
Same author

Stem nitrogen accumulation through vegetative storage proteins and mobilization to seeds supports high-yielding soybean.

Journal of experimental botany·2026

Related Experiment Video

Updated: Aug 27, 2025

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
07:42

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

7.5K

Co-Transcriptomic Analysis of the Maize-Western Corn Rootworm Interaction.

Lise Pingault1, Saumik Basu1, Neetha N Vellichirammal1

  • 1Department of Entomology, University of Nebraska-Lincoln, Lincoln, NE 68583, USA.

Plants (Basel, Switzerland)
|September 23, 2022
PubMed
Summary

Western corn rootworm (WCR) feeding triggers plant defense pathways in maize, revealing a complex transcriptomic dialogue. WCR also shows suppressed gene expression, indicating adaptive responses to resistant maize. This study illuminates plant-insect communication under pest pressure.

Keywords:
Mp708RNA-seqTranscriptomeWestern Corn Rootwormmaize

More Related Videos

Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

13.9K
Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K

Related Experiment Videos

Last Updated: Aug 27, 2025

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing
07:42

Microinjection of Western Corn Rootworm, Diabrotica virgifera virgifera, Embryos for Germline Transformation, or CRISPR/Cas9 Genome Editing

Published on: April 27, 2018

7.5K
Lateral Root Inducible System in Arabidopsis and Maize
09:23

Lateral Root Inducible System in Arabidopsis and Maize

Published on: January 14, 2016

13.9K
Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis
14:43

Lignin Down-regulation of Zea mays via dsRNAi and Klason Lignin Analysis

Published on: July 23, 2014

13.4K

Area of Science:

  • Plant Science
  • Entomology
  • Molecular Biology

Background:

  • The Western corn rootworm (WCR) is a significant pest impacting maize yield through root damage and pathogen susceptibility.
  • Plant defense responses in roots and shoots are known to be triggered by belowground herbivory, affecting intraplant communication.
  • Co-transcriptomic changes in both maize and WCR during their interaction remain largely unexplored.

Purpose of the Study:

  • To investigate the co-transcriptomic remodeling in maize and WCR interactions.
  • To elucidate the plant defense signaling network between below and aboveground tissues in a resistant maize genotype (Mp708).
  • To evaluate WCR's compensatory transcriptome responses to maize defenses.

Main Methods:

  • RNA sequencing (RNA-seq) was employed to profile the transcriptomes of maize roots and leaves.
  • Transcriptomic analysis was conducted on samples collected up to 5 days post-infestation (dpi) with WCR.
  • Gene expression patterns related to defense pathways (jasmonic acid, ethylene, benzoxazinoid) were analyzed in both maize and WCR.

Main Results:

  • Maize genotype Mp708 exhibited elevated constitutive and WCR-induced expression of jasmonic acid and ethylene pathway genes in shoots and roots.
  • Extended WCR feeding (5 days) suppressed benzoxazinoid pathway genes in Mp708 roots, a key defense mechanism in maize.
  • Downregulated genes in WCR after feeding on Mp708 roots included those involved in proteolysis, neuropeptide signaling, defense response, and hormone metabolism.

Conclusions:

  • Maize defense signaling involves dynamic transcriptomic dialog between WCR and resistant maize plants.
  • Jasmonic acid and ethylene pathways are crucial in maize's response to WCR infestation.
  • WCR exhibits compensatory transcriptomic adjustments, including suppressed gene expression, when feeding on resistant maize.