Application of deep eutectic solvents in protein extraction and purification

Area of Science:

• Green Chemistry
• Biochemistry
• Biotechnology

Background:

• Deep eutectic solvents (DESs) are tunable solvent systems composed of hydrogen bond donors and acceptors.
• DESs offer sustainable, cost-effective, and accessible properties for various chemical applications.
• Their unique physicochemical properties make them promising for extracting bioactive molecules from renewable biomass.

Purpose of the Study:

• This review focuses on the application of DESs for protein extraction and purification.
• It explores the principles and effects of DES-mediated extraction from renewable biomass.
• The study aims to highlight the potential of DESs as sustainable alternatives in protein processing.

Main Methods:

• The review synthesizes existing literature on DES applications in protein extraction.
• It discusses the mechanisms of DES-mediated extraction and purification processes.
• Analysis includes the impact of DES properties on protein recovery and stability.

Main Results:

• DESs demonstrate a significant ability to preserve the biological and functional activity of extracted proteins.
• Protein stability is enhanced in DESs compared to conventional solvents.
• DESs facilitate reproducible and scalable protein extraction and purification.

Conclusions:

• Deep eutectic solvents represent a viable and sustainable approach for protein extraction and purification.
• They offer advantages in maintaining protein integrity and enabling scalable processes.
• DESs hold potential for waste valorization in specific protein recovery applications.