Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Overview Of Cell Separation And Isolation01:20

Overview Of Cell Separation And Isolation

6.1K
Cell separation was first achieved in 1964 by S. H. Seal, who separated large tumor cells from the smaller blood cells using filtration. Two years later, Pohl and Hawk performed experiments on how cells respond differently to a nonuniform electric field based on the cell type. Such observations were the inception of cell separation methods, which allow isolating a single cell type from a heterogeneous sample.
6.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Discovery and Cryo-EM-Guided Development of a Neuropilin-2-Binding Aptamer for Receptor Antagonism.

Journal of the American Chemical Society·2026
Same author

Peptide vaccine formulations with structurally distinct STING agonist drugamers induce discrete, efficacious antitumor responses.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Rapid and label-free isolation of human peripheral blood monocytes using a reversible CD36-binding aptamer for cell capture.

Biomaterials science·2025
Same author

Investigating PolySTAT's role in clot contraction and fibrin network mechanics.

Biomaterials science·2025
Same author

Efficient and Traceless Aptamer-Based Serial Selection of Naïve and Early Memory CD8 T Cells for CAR T Cell Therapy.

Advanced healthcare materials·2025
Same author

Delivery of a fibrin-binding hemostatic polymer ameliorates neurovascular damage and neural tissue loss after traumatic brain injury.

Science advances·2025
Same journal

Removal of Codispersible Residual Impurities from CuInS<sub>2</sub>/ZnS Quantum Dots for Window-Replaceable Luminescent Solar Concentrators.

ACS applied materials & interfaces·2026
Same journal

Durable Core-Shell Scatterer Coating with Heat Storage for Radiative Cooling.

ACS applied materials & interfaces·2026
Same journal

Calix[6]arene-Based Interlocked Inverse Vulcanizate Enabling Network-Interface Cooperative Reinforcement in Natural Rubber/Carbon Black Composites.

ACS applied materials & interfaces·2026
Same journal

Resolving Thermal Accumulation and Rigid-Soft Interface Mismatch in Stretchable Electronics with Cubic Boron Nitride Composite Islands.

ACS applied materials & interfaces·2026
Same journal

Enhancing Conversion Reversibility and Initial Coulombic Efficiency of SnO<sub>2</sub> Anodes via NiO/Ni-Carbon Interfacial Design.

ACS applied materials & interfaces·2026
Same journal

Multidimensional Interface Structure Design for High-Efficiency Optically Controlled Semiconductor Devices: A Case Study on Memristive Synapses.

ACS applied materials & interfaces·2026
See all related articles

Related Experiment Video

Updated: Aug 27, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.2K

Aptamer-Based Traceless Multiplexed Cell Isolation Systems.

Emmeline L Cheng1, Nataly Kacherovsky1, Suzie H Pun1

  • 1Department of Bioengineering, University of Washington, Seattle, Washington 98195-5061, United States.

ACS Applied Materials & Interfaces
|September 23, 2022
PubMed
Summary
This summary is machine-generated.

This study introduces a novel traceless cell isolation method capable of purifying multiple cell types simultaneously using aptamers and reversal agents. This breakthrough advances cell separation for biomedical research and cell therapy manufacturing.

Keywords:
aptamercell isolationimmunomagnetic separationreversible bindingstrand displacement

More Related Videos

A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.5K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.4K

Related Experiment Videos

Last Updated: Aug 27, 2025

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells
08:30

Multiplexed Single Cell mRNA Sequencing Analysis of Mouse Embryonic Cells

Published on: January 7, 2020

13.2K
A Method of Targeted Cell Isolation via Glass Surface Functionalization
10:40

A Method of Targeted Cell Isolation via Glass Surface Functionalization

Published on: September 20, 2016

9.5K
Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
06:18

Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

Published on: March 13, 2018

14.4K

Area of Science:

  • Biotechnology
  • Molecular Biology
  • Cell Biology

Background:

  • Current cell isolation methods often require selection agents or are limited to single-cell type purification.
  • Existing traceless cell isolation techniques using aptamers can only process one cell type at a time.
  • There is a significant need for efficient methods to isolate multiple cell types simultaneously for research and clinical applications.

Purpose of the Study:

  • To develop and demonstrate a traceless, multiplexed cell isolation system capable of purifying multiple cell types concurrently.
  • To engineer and validate the simultaneous use of two distinct aptamer-reversal agent pairs for orthogonal cell elution.
  • To establish a scalable and cost-effective cell isolation platform for diverse applications.

Main Methods:

  • Engineered a CD71-binding aptamer (rvCD71apt) and its reversal agent, compatible with a previously developed CD8 aptamer (rvCD8apt) system.
  • Verified the compatibility of the two aptamer displacement mechanisms using flow cytometry.
  • Integrated rvCD71apt with a magnetic solid state for cell separation.
  • Performed sequential cell elution using orthogonal strand displacements to isolate target cell populations.

Main Results:

  • Successfully demonstrated the simultaneous traceless isolation of two distinct cell types (activated CD4+ T cells and resting CD8+ cells) from a mixed population.
  • Validated the compatibility and orthogonality of two aptamer-reversal agent systems for multiplexed cell purification.
  • Confirmed the feasibility of using aptamers with magnetic solid-state separation for efficient cell isolation.

Conclusions:

  • This study presents the first simultaneous isolation of different cell types using two aptamers and reversal agents.
  • The developed traceless multiplexed isolation system offers a promising approach for accelerating cell therapy manufacturing and biomedical research.
  • The platform has the potential for expansion to include more aptamers for isolating diverse cell types, reducing operation time and costs.