Molecularly imprinted polymer specific to creatinine complex with copper(II) ions for voltammetric determination of...

Synthesis of creatinine-imprinted polymer is challenging because of its insolubility in aprotic solvents, traditionally utilized for synthesizing molecularly imprinted polymer (MIP). Moreover, creatinine is not electroactive at conventional electrodes, and thus, introducing an electrochemical sensing platform for its determination is a difficult target. This study addressed the above-cited issues to introduce a novel creatinine voltammetric sensor with high selectivity and sensitivity. Creatinine-copper complex was found to be soluble in acetonitrile and was utilized as a template for the MIP synthesis. Methacrylic acid, ethylene glycol dimethacrylate, and azobisisobutyronitrile were used as functional monomers, cross-linker, and initiator, respectively. The MIP holding creatinine sites were used to modify the carbon paste electrode. Since creatinine did not exhibit a significant voltammetric signal, an indirect sensing technique was employed. This was based on using Cu(II) ion as an electrochemical probe. The MIP-modified electrode signal for copper ion was significantly improved in the presence of creatinine. However, the introduction of creatinine in the Cu(II) solution did not affect the NIP-modified electrode response to copper ion. The proposed sensor indicated a linear current response in the range 1 × 10^-7-1 × 10^-5 mol L^-1 with a detection limit of 5.9 × 10^-8 mol L^-1 (S/N = 3). Moreover, this method presents excellent performance in real sample analysis, with values of favorable creatinine recovery in plasma. The system exhibits acceptable precision (RSD = 4.04) and favorable selectivity toward creatinine.