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Updated: Aug 27, 2025
High-throughput Detection Method for Influenza Virus
Published on: February 4, 2012
[Apport de l'immunocapture-Elisa au diagnostic de l'infection à virus grippal A(H1N1)pdm09 : une alternative à la
Serge Védy1, Martine Valette2, Maud Bouscambert Duchamp2
1HIA Legouest, Laboratoire de biologie médicale, 27, avenue de Plantières, BP 90001 57077 Metz Cedex 3, France.
Aim Of The Study:
To evaluate immunocapture-Elisa (ICE) sensitivity versus RT-PCR in diagnosis of influenza A(H1N1)pdm09 virus infection.
Methods:
Sixty-seven RT-PCR-negative and 282 RT-PCR-positive nasopharyngeal swabs collected during winter 2009-2010 have been analyzed using ICE.
Results:
Among all the samples tested, a sensitivity of 31.3% was found for ICE. The sensitivity of ICE was directly correlated to the virus load determined through the number of cycling reactions necessary to reach detection by RT-PCR.
Conclusion:
ICE can be a suitable method compared to RT-PCR when RT-PCR cannot be used for economical or epidemiological reasons. Its sensitivity is largely dependent of the nasopharyngeal sampling quality.
