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Updated: Aug 27, 2025

Methods for Characterizing the Co-development of Biofilm and Habitat Heterogeneity
Published on: March 11, 2015
Microbial activity along the depth of biofilm in the simultaneous partial nitrification, anammox and denitrification
Xin Wen1,2, Jiansheng Huang2,3, Guoming Zeng1
1School of Civil Engineering and Architecture, Chongqing University of Science and Technology, Chongqing, People's Republic of China.
Abstract:
Simultaneous partial nitrification, anammox and denitrification (SNAD) is a sustainable and cost-effective technology for nitrogen removal from low-strength wastewater. However, knowledge of the biofilm microenvironment of the SNAD system is currently unsatisfactory. The purpose of this study was to evaluate organic carbon effects on the microenvironment and microbial growth in the SNAD biofilm system. Microelectrodes were used to investigate microbial activity in-depth within biofilms. ORP distribution of the SNAD system was positively related to anammox activity(R2 = 0.9), and had some influence on microbial community structure. The synergistic effect of anammox bacteria and denitrifiers could be achieved when the abundance ratio of anammox bacteria to denitrifying bacteria is greater than 1.2.

