Related Experiment Video
Updated: Aug 27, 2025

Preparation of Gynura bicolor DC samples for High-Resolution Tandem Mass Spectrometry
Published on: February 2, 2024
Comparative study of four Yucca species by 2D-NMR and LC-MS
Danil I Falev1, Anna V Faleva1, Ilya A Krylov2
1Core Facility Center "Arktika", Northern (Arctic) Federal University, Arkhangelsk, Russian Federation.
Abstract:
The present study was conducted to compare leaf extracts of Y. gloriosa, Y. treculeana, Y. filamentosa and Y. aloifolia. Plant extracts were obtained by sequential extraction with 50% and 100% methanol using the pressurized liquid extraction method. General comparative analysis of Yucca extracts was performed by 2 D-NMR. The presence of various biologically active components in the extracts was shown, among which steroid saponins predominate. The use of LC-MS and LC-HRMS methods with electrospray ionization made it possible to conduct a comparative analysis of Yucca steroidal saponins. The 33 steroidal saponins of various structures were found. The presence of three characteristic saponins in all Yucca species was shown: yuccaloeside C, yuccaloeside E and yuccaloeside B. The results obtained can be used to control the quality of plant materials and products.
More Related Videos
09:38Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
Published on: January 7, 2019
11:00Untargeted Metabolomics from Biological Sources Using Ultraperformance Liquid Chromatography-High Resolution Mass Spectrometry UPLC-HRMS
Published on: May 20, 2013
Related Concept Videos
High-Resolution Mass Spectrometry (HRMS)
2D NMR: Heteronuclear Single-Quantum Correlation Spectroscopy (HSQC)
2D NMR: Overview of Homonuclear Correlation Techniques
COSY90 is the standard two-dimensional (2D) COSY experiment that...
NMR Spectroscopy of Aromatic Compounds
MALDI-TOF Mass Spectrometry
Matrix-assisted laser desorption ionization (MALDI) is a commonly...
2D NMR: Overview of Heteronuclear Correlation Techniques