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Microarray analysis reveals the potential molecular mechanism of Lp299v in stable coronary atherosclerotic disease

Zhenyang Fu1, Xiaolei Song2, Anna Shen1

  • 1Department of Cardiology, The Third Affiliated Hospital of Southern Medical University, Southern Medical University, Guangzhou, 510630, China.

AMB Express
|September 24, 2022
PubMed

Insights

Lactobacillus plantarum 299v (Lp299v) supplementation impacts gene expression related to inflammation and immune response in coronary artery disease. This probiotic may influence chemokine receptors, potentially affecting cellular processes.

Area of Science:

  • Molecular biology and immunology
  • Genomics and bioinformatics
  • Cardiovascular disease research

Background:

  • Inflammatory mechanisms are crucial in coronary atherosclerotic disease (CAD) development and treatment.
  • Circulating inflammatory cytokines are elevated in CAD patients, but underlying molecular mechanisms remain unclear.
  • The role of Lactobacillus plantarum 299v (Lp299v) supplementation in modulating these mechanisms is under investigation.

Purpose of the Study:

  • To identify differentially expressed genes (DEGs) associated with Lp299v supplementation.
  • To elucidate the molecular mechanisms and identify hub genes involved in Lp299v's effects on CAD.
  • To explore the impact of Lp299v on inflammatory and immune responses.

Main Methods:

  • Utilized a microarray dataset (GSE156357) from the Gene Expression Omnibus (GEO) database.
  • Analyzed differentially expressed genes (DEGs) using R software.
  • Performed Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction (PPI) network analyses using DAVID, STRING, and Cytoscape.
  • Conducted weighted gene co-expression network analysis (WGCNA).

Main Results:

  • Identified thousands of DEGs in both daily and non-daily alcohol user groups.
  • GO analysis indicated up-regulation of miosis and down-regulation of immune response.
  • KEGG analysis revealed Lp299v reduced chemotactic cytokines and weakened immune response.
  • PPI network highlighted proteins involved in G protein-coupled receptor signaling, inflammatory response, cell proliferation, and apoptosis.
  • WGCNA identified 4 relevant DEG modules.

Conclusions:

  • Lp299v supplementation influences the expression of chemokine receptors and immune response regulation.
  • Chemokine receptors are suggested to play a cellular role in the context of Lp299v intervention.
  • Findings provide insights into the molecular effects of Lp299v in relation to inflammation and immune pathways relevant to CAD.

Keywords:
Coronary atherosclerotic diseaseG protein-coupled receptorImmune responseLactobacillus plantarum 299vMicroarray analysis

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