Instrument-Agnostizing Methodology for Liquid Chromatography-Mass Spectrometry Systems

Area of Science:

• Analytical Chemistry
• Chromatography
• Mass Spectrometry

Background:

• Mass spectrometry is vital for compound identification, quantification, and structural elucidation.
• Data transfer and comparability issues exist between different mass spectrometry instruments and analysis times.
• A need exists for instrument-independent chromatographic signal analysis.

Purpose of the Study:

• To describe a standardization procedure for mass spectrometry chromatographic signals.
• To achieve instrument-agnostic chromatographic signals.
• To enable the determination of standard retention scores for compound quantification.

Main Methods:

• Standardization of chromatographic signals from mass spectrometry platforms.
• Development of a procedure for instrument-agnostic data.
• Utilizing ultrahigh-performance liquid chromatography (UHPLC) coupled with mass spectrometry.

Main Results:

• A method for generating instrument-agnostic chromatographic signals was established.
• Standard retention scores can be determined from these signals.
• The procedure facilitates compound quantification across diverse mass spectrometry platforms.

Conclusions:

• The described standardization procedure overcomes instrument-specific limitations in mass spectrometry data.
• Instrument-agnostic signals and standard retention scores enhance cross-platform compound quantification.
• This methodology improves the reliability and comparability of results in analytical chemistry.