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Radiometric Assay of NAPE-PLD Activity
Filomena Fezza1, Nicolina Mastrangelo2, Mauro Maccarrone3,4
1Department of Experimental Medicine, Tor Vergata University of Rome, Rome, Italy. filomena.fezza@uniroma2.it.
Abstract:
N-Acyl-phosphatidylethanolamine (NAPE)-hydrolyzing phospholipase D (NAPE-PLD) is a prominent enzyme involved in the biosynthesis of fatty acid amides, a family of bioactive lipids including anandamide as the prototypical member. Here, we describe a NAPE-PLD assay based on radioactive substrates and product separation by thin layer chromatography (TLC).
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