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Updated: Aug 27, 2025

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Targeted DNA Methylation Analysis by Next-generation Sequencing
Published on: February 24, 2015
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Bioinformatics of the Endocannabinoid System: Study of DNA Methylation at Rat Cnr1 Gene Promoter
Annalaura Sabatucci1, Claudio D'Addario2
1Faculty of Bioscience and Technology for Food, Agriculture and Environment, University of Teramo, Teramo, Italy. alsabatucci@unite.it.
Methods in Molecular Biology (Clifton, N.J.)
|September 24, 2022
Abstract:
In this chapter, we will describe the bioinformatic tools that allow verifying the presence of CpG islands in a gene promoter region. We will also describe the tools needed to identify consensus motifs for specific transcription factors, focusing on the study of rat type-1 cannabinoid receptor gene (R_Cnr1) as a case study.
Keywords:
CG methylationConsensus motifsCpG islandsGenome browsersIn silico methodsRat cannabinoid receptor 1Transcription factors
