Related Experiment Video

Updated: Aug 27, 2025

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway
07:15

Mechanism of Kemeng Fang's Inhibition of Podocyte Apoptosis in Rats with Membranous Nephropathy through the PI3K/AKT Signaling Pathway

Published on: August 23, 2024

518

Florfenicol induced renal inflammatory response and apoptosis via cell adhesion molecules signaling pathway

Xiao Wang1, Wei Liu1, Ying Liu1

  • 1College of Traditional Chinese Veterinary Medicine, Hebei Agricultural University, Baoding, 071001, China.

Poultry Science
|September 24, 2022
PubMed

Insights

Florfenicol (FFC) causes kidney injury in broilers by activating the cell adhesion molecules pathway, leading to inflammation and apoptosis. This study clarifies the mechanism behind FFC-induced nephrotoxicity in poultry.

Area of Science:

  • Veterinary Medicine
  • Toxicology
  • Molecular Biology

Background:

  • Early florfenicol (FFC) use can harm broiler health, specifically causing kidney injury.
  • The precise mechanism of FFC-induced nephrotoxicity in broilers remains unclear.
  • Understanding FFC's effect on kidney signaling pathways is crucial for broiler health.

Purpose of the Study:

  • To investigate the regulatory effects of FFC on specific signaling pathways in injured broiler kidneys.
  • To explore gene and protein interactions within these pathways following FFC exposure.
  • To elucidate the molecular mechanisms underlying FFC-induced nephrotoxicity.

Main Methods:

  • Transcriptome and proteome sequencing of chick kidneys from control and FFC-treated groups.
  • Validation of differentially expressed genes and proteins using real-time quantitative PCR (qPCR) and parallel reaction monitoring (PRM).
  • Histological examination of renal tissues, measurement of inflammatory and apoptotic factors, and assessment of renal histiocyte apoptosis rate.

Main Results:

  • FFC exposure significantly altered the expression of 657 genes and 477 proteins in chick kidneys.
  • Differentially expressed genes and proteins, including CD28, ICOS, and CLDN8, were identified within the cell adhesion molecules signaling pathway.
  • FFC treatment led to increased inflammatory cell infiltration, elevated pro-inflammatory and pro-apoptotic factors, and a higher apoptotic rate in renal histiocytes.

Conclusions:

  • Florfenicol induces inflammatory reactions and apoptosis in broiler kidneys.
  • Activation of the cell adhesion molecules signaling pathway is a key mechanism in FFC-induced nephrotoxicity.
  • These findings provide critical insights into the adverse effects of FFC on poultry kidney health.
Keywords:
apoptosiscell adhesion molecules signaling pathwaychick kidneyflorfenicolinflammation

More Related Videos

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish13:25

Acute Kidney Injury Model Induced by Cisplatin in Adult Zebrafish

Published on: May 15, 2021

5.9K
Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease08:15

Author Spotlight: Network Pharmacology and Molecular Docking to Decipher the Action of Jiawei Shengjiang San Against Diabetic Kidney Disease

Published on: May 10, 2024

631

Related Concept Videos

The Extrinsic Apoptotic Pathway01:17

The Extrinsic Apoptotic Pathway

The extrinsic apoptotic pathway is initiated when extracellular death-inducing signals, such as specific cytokines, activate the death receptors expressed on the cell surface. The immune cells involved in this pathway are natural killer cells (NK cells) and cytotoxic T-lymphocytes. NK cells are critical in innate immune response, while cytotoxic T-lymphocytes are associated with adaptive immune response. These cells recognize specific receptors expressed on the altered cells and activate...
6.5K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
2.8K
Amplifying Signals via Enzymatic Cascade01:22

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze...
8.7K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.4K
NF-κB-dependent Signaling Pathway02:26

NF-κB-dependent Signaling Pathway

The transcription factor NF-κB was discovered in 1986 in the lab of Nobel laureate Professor David Baltimore, for its interaction with the immunoglobulin light chain enhancer in B-cells. After more than three decades of study, it is now evident that NF-κB regulates the expression of over 100 genes. Most of these genes play an essential role in the innate and adaptive immune responses as well as the inflammatory responses of animals.
NF-κB-dependent Signaling Mechanism
The...
7.8K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
6.7K