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Associated changes in stiffness of collagen scaffolds during osteoblast mineralisation and bone formation
Deniz Bakkalci1, Auxtine Micalet1,2, Rawiya Al Hosni1
1Division of Surgery and Interventional Science, Centre for 3D Models of Health and Disease, University College London (UCL), Charles Bell House, 43-45 Foley Street, W1W 7TS, London, UK.
Objective:
Engineering bone in 3D is important for both regenerative medicine purposes and for the development of accurate in vitro models of bone tissue. The changing material stiffness of bone tissue had not yet been monitored throughout the process of mineralisation and bone nodule formation by osteoblasts either during in vitro engineering or in development perspective.
Results:
Within this short research note, stiffness changes (Young's modulus) during in vitro bone formation by primary osteoblasts in dense collagen scaffolds were monitored using atomic force microscopy. Data analysis revealed significant stiffening of 3D bone cultures at day 5 and 8 that was correlated with the onset of mineral deposition (p < 0.00005).
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