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Isolation and Genome Analysis of Single Virions using 'Single Virus Genomics'
Published on: May 26, 2013
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Complete Genome Sequence of Bacteriophage Eula, Isolated on Microbacterium foliorum
Travis Cook1, Sophie Raffan1, Taylor Born1
1Department of Natural Sciences, Mitchell Community College, Statesville, North Carolina, USA.
Microbiology Resource Announcements
|September 26, 2022
Summary
Researchers isolated Eula, a lytic microbacteriophage, from a soil sample. This bacteriophage has a double-stranded DNA genome and was classified into cluster EB.
Area of Science:
- Microbiology
- Virology
- Genomics
Background:
- Microbacteriophages are viruses that infect bacteria.
- Bacteriophage research is crucial for understanding microbial ecosystems and developing phage-based therapies.
- Isolation and characterization of novel phages contribute to the broader knowledge of phage diversity.
Purpose of the Study:
- To isolate and characterize a novel lytic microbacteriophage from a soil environment.
- To determine the genomic features of the isolated phage.
- To classify the phage based on its genetic makeup.
Main Methods:
- Isolation of bacteriophage from a soil sample.
- Phage propagation and purification.
- Genome sequencing and analysis.
- Phylogenetic analysis based on gene content.
Main Results:
- A lytic microbacteriophage, named Eula, was successfully isolated.
- Eula infects Microbacterium foliorum NRRL B-24224.
- The Eula genome is double-stranded DNA, 41,379 base pairs in length.
- It contains 69 predicted protein-coding genes and 1 transfer RNA (tRNA) gene.
- Phylogenetic analysis placed Eula within bacteriophage cluster EB.
Conclusions:
- Eula represents a newly characterized lytic microbacteriophage.
- Its genomic data provides insights into the diversity of phages infecting Microbacterium species.
- Classification within cluster EB aids in understanding its relationship to other known bacteriophages.
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