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Updated: Aug 27, 2025

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Author Spotlight: Development of Simplified CRISPR-Based Tests for Rapid Detection of Infectious Diseases

Published on: August 16, 2024

Capturing nucleic acid variants with precision using CRISPR diagnostics.

Manoj Kumar¹, Souvik Maiti¹, Debojyoti Chakraborty¹

¹CSIR-Institute of Genomics & Integrative Biology, Mathura Road, New Delhi, 110025, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad, 201002, India.

Biosensors & Bioelectronics

|September 26, 2022

Summary

This summary is machine-generated.

CRISPR diagnostics (CRISPRDx) offer rapid, sequence-specific DNA/RNA detection and variant analysis. This review highlights CRISPR-based single nucleotide variant (SNV) detection and its diverse applications in diagnostics and therapy.

Keywords:

CRISPR/Cas CRISPRDx Mismatch sensitivity Single nucleotide variants Therapeutic applications

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Area of Science:

Molecular Biology
Biotechnology
Genomics

Background:

CRISPR/Cas systems precisely target and modify nucleotide sequences.
CRISPR-based detection has advanced molecular diagnostics for DNA/RNA targets.
Cas effectors can distinguish nucleobase variants for sequencing-free detection.

Purpose of the Study:

To review developments in CRISPR diagnostics (CRISPRDx).
To emphasize CRISPR-based single nucleotide variant (SNV) detection.
To discuss applications of CRISPRDx in various fields.

Main Methods:

CRISPR-based nucleic acid binding or cleavage for target identification.
Integration of CRISPR detection with nucleic acid amplification and signal readout.
PAM disruption-based SNV detection strategies.

Main Results:

Numerous rapid and robust CRISPR-based nucleic acid detection platforms have been developed.
CRISPRDx enables sequencing-free rapid variant detection, including SNVs.
Applications span clinical research, disease genotyping, microbial surveillance, agriculture, and industrial biotechnology.

Conclusions:

CRISPRDx offers versatile, multiplexable assays for low-cost, rapid diagnostics.
Future applications include affinity-based target sequencing and in vivo SNV detection for personalized gene therapy.
CRISPRDx holds immense potential for widespread diagnostic and therapeutic use.