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Developing a robust method integrating with selective membrane-based preconcentration and signal amplification for

Ruohong Chen1, Xingni Chen1, Hongtao Liu2

  • 1State Key Laboratory of Biocontrol, School of Life Sciences, Sun Yat-Sen University, Guangzhou, 510275, China.

Analytica Chimica Acta
|September 26, 2022
PubMed
Summary

This study presents a rapid, visual method for detecting Zika virus (ZIKV) using nanoparticle-enhanced immunoassays on a membrane. The technique accurately detects ZIKV nonstructural protein 1 (ZIKV-NS1) in complex samples within one hour.

Keywords:
Membrane-based preconcentrationProbeSignal amplificationVisible detectionZika virus

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Area of Science:

  • Biotechnology
  • Nanotechnology
  • Immunodiagnostics

Background:

  • Global concern over rapidly spreading viral infectious diseases necessitates rapid, on-site detection methods.
  • Current diagnostic tools often lack the speed and on-site capability required for timely intervention.
  • Accurate detection of specific viral proteins is crucial for disease prevention and treatment.

Purpose of the Study:

  • To develop a fast, accurate, and visual on-site method for Zika virus detection.
  • To combine virus preconcentration with nanoparticle-based signal amplification for enhanced sensitivity.
  • To validate the method's efficacy in complex biological and environmental matrices.

Main Methods:

  • Immunoassays on a membrane utilizing biotin-streptavidin scaffolds for target virus preconcentration.
  • Zika aptamer (Apt) immobilized on the membrane to capture Zika virus nonstructural protein 1 (ZIKV-NS1).
  • Gold nanoparticle-conjugated aptamer probes with horseradish peroxidase for signal amplification, visualized via a colorimetric reaction.

Main Results:

  • Achieved a detection limit of 5 ng/mL for ZIKV-NS1.
  • The entire detection procedure was completed within 1 hour.
  • Demonstrated accurate detection of ZIKV-NS1 in rabbit serum and environmental water samples, confirming matrix compatibility.

Conclusions:

  • The developed immunoassay on a membrane offers a rapid and sensitive platform for Zika virus detection.
  • The method's potential for on-site application in clinical diagnostics and environmental monitoring is significant.
  • Nanoparticle-based signal amplification and aptamer recognition provide an effective strategy for visual virus detection.