Improved Serotonin Measurement with Fast-Scan Cyclic Voltammetry: Mitigating Fouling by SSRIs
Chase Stucky1, Michael A Johnson1
1Department of Chemistry, University of Kansas, Lawrence, KS 66047 USA.
Journal of the Electrochemical Society
|September 26, 2022
Summary
Selective serotonin reuptake inhibitors (SSRIs) diminish serotonin detection signals in brain tissue studies. Using an extended waveform in fast-scan cyclic voltammetry (FSCV) can overcome this limitation.
Area of Science:
- Neuroscience
- Analytical Chemistry
- Electrochemistry
Background:
- Selective serotonin reuptake inhibitors (SSRIs) are widely used to treat serotonin-related brain disorders.
- SSRIs are also frequently employed in research involving serotonin measurement using fast-scan cyclic voltammetry (FSCV).
Purpose of the Study:
- To investigate the impact of common SSRIs on serotonin detection via FSCV.
- To evaluate electrode modifications and waveform strategies for improving FSCV measurements in the presence of SSRIs.
Main Methods:
- Fast-scan cyclic voltammetry (FSCV) was used to measure serotonin.
- Three SSRIs (fluoxetine, escitalopram, sertraline) were tested with carbon-fiber microelectrodes (CFMs).
- Electrode performance was assessed with both bare CFMs and Nafion-coated CFMs using standard and extended waveforms.
Main Results:
- SSRIs significantly reduced serotonin oxidation current with the standard Jackson waveform.
- Nafion coating further degraded CFM performance, increasing response times and reducing currents.
- An extended waveform (+1.3 V) with bare CFMs mitigated current reduction and electrode fouling.
Conclusions:
- Electrode fouling is a critical consideration in FSCV experiments involving SSRIs.
- The selection of an appropriate waveform is essential for accurate serotonin detection in the presence of SSRIs.
- The extended waveform offers a viable solution to overcome SSRI-induced signal degradation in FSCV.
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