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Related Experiment Video

Updated: Aug 27, 2025

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Changes in aging-induced kidney dysfunction in mice based on a metabolomics analysis.

Danli Jiao1, Li Qi2, Li Hu1

  • 1School of Acupuncture-Moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai, China.

Frontiers in Endocrinology
|September 26, 2022
PubMed
Summary

Aging impairs kidney function, leading to inflammation and fibrosis. Metabolomics identified key metabolic pathways and potential biomarkers in urine, offering insights into therapeutic targets for age-related kidney dysfunction.

Keywords:
biomarkerskidney dysfunctionnatural aginguntargeted metabolomicsurine

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Area of Science:

  • Gerontology and Nephrology
  • Metabolomics and Systems Biology

Background:

  • Kidney dysfunction is a significant consequence of systemic organ aging.
  • Understanding the metabolic mechanisms underlying age-related kidney injury is crucial.

Purpose of the Study:

  • To investigate the metabolic alterations in aging kidneys using metabolomics.
  • To identify key metabolic pathways and potential biomarkers associated with kidney dysfunction in aging mice.

Main Methods:

  • Analysis of serum biochemistry and kidney histopathology in young (3-month) and aged (24-month) Kunming mice.
  • High-resolution untargeted metabolomics on kidney and urine samples.
  • Pathway analysis of differential metabolites.

Main Results:

  • Aged mice exhibited inflammatory lesions, fibrosis, and impaired kidney function.
  • Significant changes in 25 kidney and 20 urine metabolites were observed in aged mice.
  • Six key metabolic pathways (including TCA cycle, purine metabolism) were identified as significantly altered.
  • Nine urinary metabolites were identified as potential biomarkers for aging-related kidney dysfunction.

Conclusions:

  • Aging significantly impacts kidney function through alterations in amino acid, carbohydrate, and nucleotide metabolism.
  • These metabolic changes contribute to inflammation, oxidative stress, and bioenergy deficits in aging kidneys.
  • Identified metabolites and pathways serve as potential therapeutic targets and risk assessment biomarkers for senile kidney injury.