Sustainable treatment of nitrate-containing wastewater by an autotrophic hydrogen-oxidizing bacterium

Bacteria are key denitrifiers in the reduction of nitrate (NO₃ ^--N), which is a contaminant in wastewater treatment plants (WWTPs). They can also produce carbon dioxide (CO₂) and nitrous oxide (N₂O). In this study, the autotrophic hydrogen-oxidizing bacterium Rhodoblastus sp. TH20 was isolated for sustainable treatment of NO₃ ^--N in wastewater. Efficient removal of NO₃ ^--N and recovery of biomass nitrogen were achieved. Up to 99% of NO₃ ^--N was removed without accumulation of nitrite and N₂O, consuming CO₂ of 3.25 mol for each mole of NO₃ ^--N removed. The overall removal rate of NO₃ ^--N reached 1.1 mg L^-1 h^-1 with a biomass content of approximately 0.71 g L^-1 within 72 h. TH20 participated in NO₃ ^--N assimilation and aerobic denitrification. Results from ¹⁵N-labeled-nitrate test indicated that removed NO₃ ^--N was assimilated into organic nitrogen, showing an assimilation efficiency of 58%. Seventeen amino acids were detected, accounting for 43% of the biomass. Nitrogen loss through aerobic denitrification was only approximately 42% of total nitrogen. This study suggests that TH20 can be applied in WWTP facilities for water purification and production of valuable biomass to mitigate CO₂ and N₂O emissions.