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A new system for multiplexed mosaic analysis of gene function in the mouse
Hongchen Cai1, Monte M Winslow1,2
1Department of Genetics, Stanford University School of Medicine, Stanford, CA, USA.
Cell Reports Methods
|September 26, 2022
Summary
Researchers developed a novel mouse model for studying gene function. This system uses Cre/lox technology to create genetic mosaicism, allowing for the simultaneous analysis of multiple genes in living organisms.
Area of Science:
- Genetics and Genomics
- Molecular Biology
- Animal Models
Background:
- Understanding gene function is crucial for biological and medical research.
- Existing methods for studying gene function in vivo can be limited in scale and multiplexing capability.
Purpose of the Study:
- To present an innovative mouse model for assessing gene function.
- To enable the multiplexed assessment of gene function in somatic cells in vivo.
Main Methods:
- Development of a Cre/lox-based mouse model.
- Stochastic, single-sgRNA expression in somatic cells.
- Generation of genetic mosaicism for multiplexed gene function analysis.
Main Results:
- Successful creation of a mouse model enabling multiplexed gene function assessment.
- Demonstration of stochastic transgenic expression of up to 100 sgRNAs.
- Establishment of genetic mosaicism for in vivo studies.
Conclusions:
- The developed mouse model offers a powerful tool for large-scale genetic screens.
- This system facilitates the study of gene function in a multiplexed manner in vivo.
- The innovation advances the field of functional genomics research.
