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Microarray analysis of lncRNA and mRNA expression profiles in patients with Legg-Calve-Perthes disease
Shangyu Wang1, Haobo Zhong2, Renhao Ze1
1Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Insights
Legg-Calve-Perthes disease (LCPD) pathogenesis remains unclear. This study identified differentially expressed long non-coding RNAs (lncRNAs) and messenger RNAs (mRNAs) in LCPD tissues, revealing potential molecular mechanisms and targets for future research.
Area of Science:
- Molecular Biology
- Genomics
- Pathogenesis Research
Background:
- The exact causes and mechanisms of Legg-Calve-Perthes disease (LCPD) are not fully understood.
- Ischemic necrosis due to disrupted blood supply to the femoral head is considered a key event in LCPD.
- The role of long non-coding RNAs (lncRNAs) in LCPD development has not been previously investigated.
Purpose of the Study:
- To profile differentially expressed lncRNAs and mRNAs in LCPD patients.
- To construct lncRNA-mRNA interaction networks to identify potential regulatory targets in LCPD.
- To explore the molecular mechanisms underlying LCPD development.
Main Methods:
- Microarray analysis was used to identify differentially expressed lncRNAs and mRNAs in LCPD tissues.
- Gene Ontology (GO) terms and pathways related to vascular function and coagulation were analyzed.
- lncRNA-mRNA interaction networks were constructed and validated using qRT-PCR.
Main Results:
- Microarray analysis identified significant numbers of up-regulated and down-regulated lncRNAs and mRNAs in LCPD tissues.
- An mRNA-lncRNA interacting network was constructed, identifying 13 differentially expressed lncRNAs potentially involved in LCPD pathogenesis.
- Validation confirmed the differential expression of selected mRNAs and lncRNAs.
Conclusions:
- This study established a co-expression network of lncRNAs and mRNAs associated with LCPD.
- The findings provide novel insights into the molecular mechanisms of LCPD.
- Identified lncRNAs and mRNAs represent potential targets for future LCPD research.
Background:
The etiology and underlying pathogenic mechanisms of Legg-Calve-Perthes disease (LCPD) still remain unclear. A disruption of blood supply to the femoral head, producing ischemic necrosis, appears to be the critical pathological event. The lncRNAs play crucial roles in many biological processes and are dysregulated in various human diseases. However, its expression profiles and the potential regulatory roles in the development of LCPD have not been investigated.
Methods:
In this study, differentially expressed lncRNA and mRNA of Legg-Calve-Perthes disease patients were profiled. Several GO terms and pathways that play important roles in the regulation of vascular structure, function or coagulation were selected for further analysis. The lncRNA -mRNA interacting networks in LCPD tissues were constructed to identify novel potential targets for further investigation.
Results:
The microarray analysis revealed that 149 lncRNAs and 37 mRNAs were up-regulated, and 64 lncRNAs and 250 mRNAs were down-regulated in LCPD tissues. After filtering, we finally found 14 mRNAs and constructed an mRNA-lncRNA interacting network. Through the analysis of the interaction network, we finally found 13 differentially expressed lncRNAs, which may be implicated in the pathogenesis of LCPD. These mRNAs/lncRNAs were further validated with qRT-PCR.
Conclusion:
The findings of this study established a co-expression network of disease-related lncRNAs and mRNAs which screened out from the concerned G.O. terms and Pathways, which may provide new sights for future studies on molecular mechanisms of LCPD.
