[Perfusion of the dog tibia isolated for the study of mineral metabolism]

A method for perfusion of isolated canine tibia is described. With this procedure the influence of different albumins on the uptake of tritiated 25-hydroxyvitamin D3 (3H-25(OH)D3) by the tibia has been studied. Bovine serum albumin at 1% concentration seems to be optimum for this system. This experimental approach seems to be useful for the study of mineral metabolism.