Deinococcus taeanensis sp. nov., a Radiation-Resistant Bacterium Isolated from a Coastal Dune

A Gram-stain-negative, nonspore-forming, nonmotile, aerobic, rod-shaped, and very pale orange-colored bacterial strain, designated TS293^T, was isolated from a sand sample obtained from a coastal dune after exposure to 3kGy of gamma (γ)-radiation. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that the isolate was a member of the genus Deinococcus and clustered with D. deserti VCD115^T. The genome of strain TS293^T was 4.62 Mbp long (68.2% G + C content and 4124 predicted genes) divided into a 2.86Mb main chromosome and five plasmids. Many genes considered to be important to the γ-radiation and oxidative stress resistance of Deinococcus were conserved in TS293^T, but genome features that could differentiate TS293^T from D. deserti and D. radiodurans, the type species of the Deinococcus genus, were also detected. Strain TS293^T showed resistance to γ-radiation with D₁₀ values (i.e., the dose required to reduce the bacterial population by tenfold) of 3.1kGy. The predominant fatty acids of strain TS293^T were summed feature 3 (C_16:1 ω6c and/or C_16:1 ω7c) and iso-C_16:0. The major polar lipids were two unidentified phosphoglycolipids and one unidentified glycolipid. The main respiratory quinone was menaquinone-8. Based on the phylogenetic, genomic, physiological, and chemotaxonomic characteristics, strain TS293^T represents a novel species, for which the name Deinococcus taeanensis sp. nov. is proposed. The type strain is TS293^T (= KCTC 43191^T = JCM 34027^T).