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Combined Use of Tail Vein Metastasis Assays and Real-Time In Vivo Imaging to Quantify Breast Cancer Metastatic Colonization and Burden in the Lungs

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Monitoring Angiotropic Extravascular Migratory Metastasis In Vitro.

Claire Lugassy¹, Hynda K Kleinman², Raymond L Barnhill^3,4

¹Department of Translational Research, Institut Curie, Paris, France. claire.lugassy@curie.fr.

Methods in Molecular Biology (Clifton, N.J.)

|September 26, 2022

Summary

This summary is machine-generated.

This study introduces a simple 3D assay to observe cancer cell metastasis. The method tracks cancer cell migration along blood vessels, aiding in understanding and potentially blocking cancer spread.

Keywords:

Angiotropism Cancer Extravascular migratory metastasis (EVMM)In vitro 3D coculture Melanoma Pericytic mimicry Real time monitoring Tumor migration

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Area of Science:

Oncology
Cell Biology
Biotechnology

Background:

Cancer metastasis remains a poorly understood process, limiting effective treatment strategies.
Extravascular migratory metastasis (EVMM) is a recognized pathway where tumor cells migrate along vascular surfaces.
Existing methods for studying metastasis lack real-time, quantitative analysis of cell-vessel interactions.

Purpose of the Study:

To develop a simple, quantitative in vitro 3D assay for studying cancer cell migration along vascular structures.
To monitor cancer cell migration dynamics and phenotypic switching in real-time.
To provide a platform for molecular analysis and therapeutic agent screening for metastasis.

Main Methods:

A novel 3D co-culture assay based on an angiogenesis assay was developed.
Fluorescence-tagged cancer cells were monitored in real-time alongside vascular structures.
The assay quantifies cancer cell migration along the abluminal surface of vessels.

Main Results:

The assay successfully monitors single cancer cell migration along vascular structures in real time.
It demonstrates the ability to observe phenotypic switching and migration dynamics.
The protocol is effective for quantitative analysis of extravascular migratory metastasis.

Conclusions:

The developed 3D assay offers a simple and quantitative method to study cancer cell metastasis.
This assay can be utilized for in-depth molecular investigations of metastatic processes.
The platform shows potential for screening novel therapeutic agents to inhibit cancer spread.