Related Experiment Video
Updated: Aug 27, 2025
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
Published on: November 30, 2009
The Chick CAM as an In Vivo System to Study Stem Cell Activity
Marta Teixeira Pinto1,2,3, Ana Sofia Ribeiro4,5, Joana Paredes4,6,5,7
1i3S-Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal. mtpinto@i3s.up.pt.
Abstract:
The chick embryo chorioallantoic membrane (CAM), an extensively vascularized extraembryonic membrane, has been widely used to study several aspects of tumor development including tumor-induced angiogenesis, tumor cell proliferation, and metastasis. Based on the tumor cell/CAM system, we focused here on the identification and quantification of cancer stem cells. We validated the CAM model as a suitable model to evaluate stem cell activity in a given mixed cell population.
