Aptamer-based biosensors for Pseudomonas aeruginosa detection

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Microbiology

Background:

• Pseudomonas aeruginosa exhibits innate antibiotic resistance, posing a significant global health threat.
• Carbapenem-resistant strains are a top priority on the WHO's list of antimicrobial-resistant hazards.
• Early detection of Pseudomonas aeruginosa is crucial for effective treatment and infection control.

Purpose of the Study:

• To review recent advancements in aptamer-based biosensors for detecting Pseudomonas aeruginosa.
• To highlight the application of aptamer biosensors in point-of-care diagnostics.
• To summarize progress in optical, electrochemical, and piezoelectric signal transduction methods for P. aeruginosa detection.

Main Methods:

• Literature review focusing on aptamer biosensor development for Pseudomonas aeruginosa detection.
• Analysis of studies employing optical, electrochemical, and piezoelectric signal transduction.
• Synthesis and characterization of aptamers as biorecognition elements.

Main Results:

• Aptamers show comparable affinity and specificity to antibodies for pathogen recognition.
• Biosensors offer advantages in speed, cost, sensitivity, and selectivity for pathogen detection.
• Significant progress has been made in aptamer biosensor development for P. aeruginosa since 2015.

Conclusions:

• Aptamer-based biosensors are a promising technology for the rapid and sensitive detection of Pseudomonas aeruginosa.
• These biosensors facilitate point-of-care diagnostics, aiding in early identification and treatment.
• Continued research in signal transduction methods will further enhance diagnostic capabilities.