Effect of the Sargassum angustifolium Extract on Methamphetamine-Induced Cytotoxicity in SH-SY5Y Cells

This study aimed to assess the effect of the Sargassum angustifolium extract in methamphetamine-induced SH-SY5Y cells death. The brown algae S. angustifolium was extracted with 80% ethanol. The SH-SY5Y cells were treated with different concentrations of methamphetamine to measure IC₅₀.. The MTT test was used to assess the toxic effect of the S. angustifolium extract in SH-SY5Y cells. SH-SY5Y cells' survival was measured while cells were treated with different concentrations of methamphetamine and S. angustifolium extract simultaneously. A specific kit measured intracellular ROS levels. Western blot analysis evaluated the expression of cytochrome C and Bax/Bcl2 ratio. The results showed that 5 mM methamphetamine approximately killed 50% of the cells, so it is considered IC₅₀. The MTT test showed no toxicity effect for the S. angustifolium extract. 80, 160, 320, and 640 μg/ml of S. angustifolium extract prevented the occurrence of methamphetamine toxic effects in SH-SY5Y cells after 24 hours. Moreover, the S. angustifolium extract decreased ROS levels and cytochrome C release and reduced BaX/Bcl2 ratio in cells treated by methamphetamine. On the whole, it seems that the S. angustifolium hydroalcoholic extract has the potential to increase cell survival through in vitro antioxidant and antiapoptotic activities.