Related Experiment Video
Updated: Aug 27, 2025
Dynamic Monitoring of Seroconversion using a Multianalyte Immunobead Assay for Covid-19
Published on: February 16, 2022
[Evaluation of two immunocromatographic tests for the detection of antibodies against SARS-CoV-2]
S Medrano1, M Martínez-Rodríguez, L Vallejo
1Sara Medrano Pardo, Microbiology Department. Hospital Clínico San Carlos. C/ Martín Lago s/n. 28040-Madrid. Spain. smedranopardo@gmail.com.
Objective:
Serological tests have been a valuable tool during the SARS-CoV-2 pandemic, supporting molecular methods for detection, and monitoring the immune response, caused by vaccination or by natural infection. Within all these techniques, rapid tests are interesting due to their ease of use, rapid response and low cost.
Methods:
Two different immunological techniques were evaluated: Realy Tech and Mikrogen Diagnostik recomLine SARS-CoV-2 IgG. SARS-CoV-2 IgG II Quant antibody test and SARS-CoV-IgG assay, both from Abbott Diagnostics, were used as reference techniques.
Results:
Mikrogen Diagnostik recomLine SARS-CoV-2 IgG shows the best results (S=0.985; E=0.839). Three techniques offered good positive predictive values, but Realy Tech and Healgen negative predictive values left to be desired.
Conclusions:
Mikrogen Diagnostik recomLine SARS-CoV-2 IgG showed good results in the detection of antibodies against SARS-CoV-2 and could be used as an alternative to automated techniques.
