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Updated: Aug 27, 2025

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
Polyelectrolyte-grafted anion exchangers with hydrophilic intermediate layers for ion chromatography
Kai Zhang1, Yonggang Zhao2, Mingli Ye2
1School of Life Sciences and Materials Chemistry, College of Science and Technology Ningbo University, Ningbo 315300, China.
Abstract:
The hydrophobicity of polymer-based anion exchangers is a persistent problem in suppressed anionic chromatography (SAC) due to its adverse effect on chromatographic performance. Herein we describe polyelectrolyte-grafted anion exchangers with modified hydrophilic intermediate layers. The anion exchangers were functionalized by successively grafting a linear cationic condensation polymer (LCCP), a preprepared polyelectrolyte. The carboxylic/hydroxylic intermediate layers formed during thiol-radical-mediated polymerization exert distinct effects on the phase capacity and hydrophobicity. The separation of typical inorganic anions, polarizable anions, and organic acids shows that the anion exchangers display good performance in SAC mode.
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