A low-cost in-situ bioremediation process for perchlorate contaminated aqueous phase

Perchlorate is a known endocrine-disrupting micropollutant. The efficiency of a low-cost in-situ bio-remediation process for perchlorate-contaminated aqueous phase was evaluated in a bench-scale unit in this study. The two-stage process unit comprises an anaerobic leach bed unit (5.3 L) for generating leachate and an anaerobic filter bed unit (10 L) inoculated with an isolated perchlorate reducing Serratia marcescens (GenBank Accession No. JQ807993). Organic leachate produced from anaerobic digestion of vegetable waste served as a sole substrate for the perchlorate reduction, and needle-felt natural fibre was used as a filter bed medium. The filter bed unit removed 98.5% of perchlorate at 10 mg/L initial concentration (volumetric loading, 39 mg/L/day) at an optimal soluble COD concentration of 40 mg/L in the leachate and a hydraulic retention time of 6.15 h. Controlled leachate delivery results in an effluent COD < 20 mg/L, reducing the risk of residual organic contamination in the treated water. Considering the many advantages, this approach would be more feasible for treating perchlorate-contaminated aquifers, streams, and surface canals.