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Microbial mediated arsenate reducing behavior in landfill leachate-saturated zone
Jinbao Liu1, Dongchen Zhang2, Yongjun Luo3
1Zhejiang Tongji Vocational College of Science and Technology, Hangzhou, 311231, China.
Microbial reduction of arsenic(V) in landfill leachate sludge (LSZ) enhances arsenic resistance. Microbial diversity and arsenic-reducing genes (arrA, arsC) varied with arsenic content and nutrient metabolism.
Area of Science:
- Environmental Microbiology
- Geochemistry
- Bioremediation
Background:
- Arsenic(V) is a major toxic species in landfills, necessitating microbial resistance mechanisms.
- Landfill leachate sludge (LSZ) is a potential environment for microbial arsenic transformation.
- Microbial arsenic(V) reduction is crucial for mitigating arsenic toxicity in landfill environments.
Purpose of the Study:
- To investigate microbial arsenic(V)-reducing behavior in LSZ.
- To analyze the relationship between microbial community structure, functional genes, and arsenic(V) content.
- To model landfill arsenic bio-reduction pathways and their correlation with nutrient cycling.
Main Methods:
- Analysis of microbial arsenic(V) reduction efficiency under varying arsenic(V) concentrations.
- Microbial community structure analysis using functional gene distribution (arrA, arsC).
- Modeling of arsenic bio-reduction pathways and correlation with C, N, and S metabolic processes.
Main Results:
- Higher arsenic(V) reduction efficiency observed in LSZ with higher arsenic(V) content.
- Microbial diversity and community structure correlated with arsenic(V) levels and functional gene distribution.
- Nutrient metabolic processes, including thiosulfate oxidation, denitrification, and dissimilatory nitrate reduction, positively influenced arsenic resistance genes.
Conclusions:
- Microbial arsenic(V) reduction in LSZ is influenced by arsenic(V) concentration and microbial community composition.
- Functional genes arrA and arsC play key roles in arsenic(V) bio-reduction within landfills.
- Nutrient element metabolic processes significantly impact the distribution of arsenic resistance genes, offering insights into landfill arsenic biogeochemistry.
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