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Engineered biomaterials to guide spheroid formation, function, and fabrication into 3D tissue constructs.

Nikolas Di Caprio1, Jason A Burdick2

  • 1Department of Bioengineering, University of Pennsylvania, Philadelphia, PA 19104, USA.

Acta Biomaterialia
|September 27, 2022
PubMed
Summary

Engineered biomaterials guide cellular spheroid formation and function for advanced tissue engineering. This review highlights strategies for creating tissue models and therapeutic applications using biofabrication techniques.

Keywords:
BiofabricationBiomaterialBioprintingHydrogelScaffoldsSpheroidTissue engineering

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Cell Biology

Background:

  • Cellular spheroids offer advantages over traditional cell cultures for mimicking tissue structures.
  • However, uncontrolled growth and simplicity limit their use in complex applications.
  • Biomaterials are crucial for overcoming these limitations and expanding spheroid applications.

Purpose of the Study:

  • To review recent advances in using engineered biomaterials to guide spheroid formation and function.
  • To explore the fabrication of spheroids into functional tissue constructs for biomedical applications.
  • To provide an outlook on future directions in biomaterial-guided spheroid development.

Main Methods:

  • Overview of biomaterial strategies for high-throughput, homogeneous spheroid fabrication.
  • Summary of internal (microparticles) and external (hydrogels) biomaterial incorporation to influence spheroid behavior.
  • Discussion of biofabrication techniques (3D bioprinting, melt electrowriting) for assembling spheroids into macroscale tissues.

Main Results:

  • Engineered biomaterials enable controlled spheroid formation and enhanced function (e.g., differentiation, fusion).
  • Biofabrication methods allow the guided assembly of spheroids into complex tissue models and implantable constructs.
  • Biomaterials provide structural support and guide spheroid development for diverse biomedical applications.

Conclusions:

  • Engineered biomaterials are pivotal in advancing the use of cellular spheroids in tissue engineering and regenerative medicine.
  • Biofabrication approaches combined with biomaterials offer powerful tools for creating sophisticated tissue models and therapies.
  • Future research will focus on novel biomaterials to further enhance biofabricated spheroid-based constructs.