Jove
Visualize
Contact Us

Related Concept Videos

General Transcription Factors01:30

General Transcription Factors

5.5K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
5.5K
Ribosome Profiling02:24

Ribosome Profiling

3.6K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
3.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Reference protein-coding transcripts of human genes annotated using long-read transcriptome datasets.

Scientific reports·2026
Same author

A visualization tool for individual gene expression profiles among males and females in GTEx tissues.

Biology of sex differences·2025
Same author

Housekeeping protein-coding genes interrogated with tissue and individual variations.

Scientific reports·2024
Same author

Dominant transcript expression profiles of human protein-coding genes interrogated with GTEx dataset.

Scientific reports·2022
Same author

Top-ranked expressed gene transcripts of human protein-coding genes investigated with GTEx dataset.

Scientific reports·2020
Same author

Overlapping protein-coding genes in human genome and their coincidental expression in tissues.

Scientific reports·2019
Same journal

Development of CypriSSR: a genome-wide, chromosome-level microsatellite database for multiple cyprinidae species.

Database : the journal of biological databases and curation·2026
Same journal

KitBase Expanded: An Integrated Genomic and Phenotypic Resource for 3,268 Fast-Neutron-Irradiated Rice Mutants.

Database : the journal of biological databases and curation·2026
Same journal

PhaLP 2.0: extending the community-oriented phage lysin database with a SUBLYME pipeline for metagenomic discovery.

Database : the journal of biological databases and curation·2026
Same journal

A similarity metric, rubric, and unified hierarchy for biomedical publication types and study designs.

Database : the journal of biological databases and curation·2026
Same journal

GUTAID: a curated database linking gut microbial antigens to autoimmune mechanisms.

Database : the journal of biological databases and curation·2026
Same journal

Rosetta Statements: simplifying FAIR knowledge graph construction with a user-centred approach.

Database : the journal of biological databases and curation·2026
See all related articles
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Aug 27, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.7K

TEx-MST: tissue expression profiles of MANE select transcripts.

Kuo-Feng Tung1, Wen-Chang Lin1,2

  • 1Institute of Biomedical Sciences, Academia Sinica, Taipei 115, Taiwan, R.O.C.

Database : the Journal of Biological Databases and Curation
|September 28, 2022
PubMed
Summary
This summary is machine-generated.

A new database, TEx-MST, provides tissue expression profiles for human protein-coding genes. It integrates data from the Matched Annotation from the NCBI and EMBL-EBI (MANE) select transcripts and the Genotype-Tissue Expression (GTEx) project.

More Related Videos

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
08:33

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

12.2K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K

Related Experiment Videos

Last Updated: Aug 27, 2025

Cell Type-specific Gene Expression Profiling in the Mouse Liver
10:06

Cell Type-specific Gene Expression Profiling in the Mouse Liver

Published on: September 17, 2019

7.7K
Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches
08:33

Dissection of Drosophila melanogaster Flight Muscles for Omics Approaches

Published on: October 17, 2019

12.2K
Identification of Alternative Splicing and Polyadenylation in RNA-seq Data
08:35

Identification of Alternative Splicing and Polyadenylation in RNA-seq Data

Published on: June 24, 2021

5.7K

Area of Science:

  • Genomics
  • Bioinformatics
  • Gene Expression Analysis

Background:

  • The Matched Annotation from the NCBI and EMBL-EBI (MANE) select project established unified representative transcripts for human protein-coding genes.
  • However, explicit tissue expression data for these MANE select transcripts were lacking, hindering functional interpretation.

Purpose of the Study:

  • To provide comprehensive tissue expression profiles for MANE select transcripts across various normal human tissues.
  • To create a user-friendly web database (TEx-MST) for exploring these expression patterns and their functional significance.

Main Methods:

  • Utilized the Genotype-Tissue Expression (GTEx) V9 dataset, based on long-read sequencing, for enhanced assessment of alternative splicing.
  • Integrated MANE select transcript data with GTEx expression data, cross-referencing with GENCODE biotype information.
  • Developed a web-based bioinformatics database (TEx-MST) with search and browsing functionalities.

Main Results:

  • A total of 18,083 genes showed overlap between MANE and GTEx datasets.
  • 13,245 MANE select transcripts were identified as top-ranked, highly expressed transcripts in the GTEx V9 dataset, confirming their dominant expression.
  • The TEx-MST database offers a visualized interface for normal tissue expression patterns of MANE select transcripts.

Conclusions:

  • The TEx-MST database successfully integrates MANE select transcript information with GTEx expression data, providing valuable insights into human gene expression.
  • This resource facilitates the study of molecular modulations and functional significance of MANE select transcripts in normal human tissues.
  • The database serves as a key resource for researchers investigating gene expression patterns and alternative splicing in human health and disease.