AFB1 recognition from liver tissue via AFB1 imprinted magnetic nanoparticles

Area of Science:

• Materials Science
• Analytical Chemistry
• Biotechnology

Background:

• Aflatoxins (AFs), particularly aflatoxin B1 (AFB1), are highly toxic and carcinogenic compounds produced by Aspergillus species.
• Accurate recognition and quantification of AFB1 in food and biological samples are crucial for public health.
• Existing detection methods may lack selectivity or efficiency, necessitating the development of advanced materials.

Purpose of the Study:

• To synthesize and characterize Poly(HEMA) based imprinted magnetic nanoparticles (magAFB1-MIPs) for the selective recognition of AFB1.
• To evaluate the adsorption capacity, selectivity, and reusability of the synthesized magAFB1-MIPs.
• To assess the performance of magAFB1-MIPs for AFB1 removal from spiked liver tissue samples.

Main Methods:

• Synthesis of magAFB1-MIPs and non-imprinted polymers (NIPs) using varying mole ratios.
• Characterization using swelling tests, surface area measurements, SEM, and particle size analysis.
• Adsorption studies to determine capacity and selectivity, followed by reusability tests and application in spiked liver tissue.

Main Results:

• Synthesized magAFB1-MIPs exhibited a high surface area (117 m²/g) and particle size (483 nm).
• High template removal (91%) and polymerization yield (98%) were achieved.
• Maximum adsorption capacities were 427.57 ng/g for magAFB1-MIPs versus 44.6 ng/g for magNIPs.
• Demonstrated selectivity for AFB1 over other aflatoxins (AFB2, AFG1, AFG2).
• Satisfactory AFB1 removal from spiked liver tissue samples was observed.
• Negligible decrease in adsorption capacity over 10 adsorption-desorption cycles indicated good reusability.

Conclusions:

• The developed magAFB1-MIPs are effective for selective AFB1 recognition due to their high adsorption capacity and selectivity.
• These nanoparticles show potential for practical applications in detecting AFB1 in complex matrices like liver tissue.
• The reusability of the magnetic nanoparticles suggests a cost-effective and sustainable approach for AFB1 analysis.