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Related Experiment Videos

Serial sectioning techniques for a modified LKB Historesin.

E C Yeung, S K Law

    Stain Technology
    |May 1, 1987
    PubMed
    Summary
    This summary is machine-generated.

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    Adding polyethylene glycol (PEG) to LKB Historesin embedding medium significantly improves serial section ribbon formation. Optimal PEG concentration and microtome type are crucial for successful serial sectioning of tissues.

    Area of Science:

    • Histology
    • Materials Science

    Background:

    • LKB introduced a new glycol methacrylate-based plastic for serial sectioning.
    • The LKB Historesin Embedding Kit (2218-500) requires evaluation for its ribbon-forming capacity.

    Purpose of the Study:

    • To determine optimal conditions for serial section ribbon formation using LKB Historesin.
    • To assess the impact of polyethylene glycol 400 (PEG) concentration and block preparation on ribbon quality.

    Main Methods:

    • Tested various block sizes, PEG concentrations, and tissue types.
    • Examined ribbon formation using different microtomes (conventional and with retractable specimen arm).
    • Evaluated the effect of adding PEG and dental wax to the embedding medium and block surfaces.

    Main Results:

    Related Experiment Videos

    • Unmodified Historesin produced fragile ribbons.
    • Addition of PEG enhanced ribbon formation, with optimal results at 0.2 ml PEG/5.0 ml medium using glass knives.
    • Serial sectioning with conventional microtomes was improved by adding PEG (0.25-0.30 ml/5 ml) and dental wax.
    • Microtomes with retractable specimen arms facilitated easier ribbon formation.

    Conclusions:

    • Polyethylene glycol (PEG) is essential for improving ribbon formation in LKB Historesin.
    • Specific concentrations of PEG and microtome features are critical for successful serial sectioning.
    • Parallel block faces are vital for producing continuous serial sections.