Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Lipoprotein(a)-associated proteomic signature predicts cardiovascular disease in young adults.

The Journal of clinical investigation·2026

Same author

Plasma von Willebrand Factor and ADAMTS13 Interact With <i>APOE</i>-ε4 in Predicting Longitudinal Brain Atrophy and Cognitive Decline Over a 9-Year Follow-Up.

Journal of the American Heart Association·2026

Same author

Multi-parametric interferometric reflectance imaging sensor.

Scientific reports·2026

Same author

A multi-layered approach to elucidate mechanisms of physical function in response to rehabilitation in heart failure with preserved ejection fraction.

medRxiv : the preprint server for health sciences·2026

Same author

Proteomic signatures of pediatric cardiovascular and cardiometabolic traits demonstrate long-term modifiable drivers of adult disease.

medRxiv : the preprint server for health sciences·2025

Same author

Matrix metalloproteinase-1 and interleukin-17A are associated with previous plaque disruption: a combined proteomics and optical coherence tomography study.

Coronary artery disease·2025

Search research articles

Related Experiment Video

Updated: Aug 27, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

Attomolar sensitivity microRNA detection using real-time digital microarrays.

Fulya Ekiz Kanik¹, Iris Celebi¹, Derin Sevenler^1,2

¹Department of Electrical and Computer Engineering, Boston University, Boston, MA, USA.

Scientific Reports

|September 28, 2022

Summary

This study introduces a novel digital microarray for highly sensitive microRNA detection. The new method uses plasmonic gold nanorods and real-time particle tracking to achieve attomolar detection limits, improving diagnostic capabilities for diseases.

More Related Videos

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Related Experiment Videos

Last Updated: Aug 27, 2025

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs

Published on: April 14, 2015

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs

Published on: August 3, 2011

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Circulating MicroRNA Quantification Using DNA-binding Dye Chemistry and Droplet Digital PCR

Published on: June 26, 2016

Area of Science:

Molecular Biology
Nanotechnology
Biomedical Diagnostics

Background:

MicroRNAs (miRNAs) are crucial noncoding RNAs implicated in various diseases, including cancer and cardiovascular disorders.
Accurate miRNA detection is vital for diagnostics, but current methods lack sufficient sensitivity and specificity.
DNA microarray technology offers high throughput but faces limitations in miRNA analysis.

Purpose of the Study:

To develop a highly sensitive and multiplexed digital microarray for miRNA detection.
To enhance sensitivity and specificity beyond traditional end-point assays.
To enable earlier and more accurate disease diagnosis through improved biomarker detection.

Main Methods:

Development of a digital microarray utilizing plasmonic gold nanorods as labels.
Implementation of real-time particle tracking for target molecule monitoring on the sensor surface.
Proof-of-concept studies using miRNA-451a and miRNA-223-3p detection.

Main Results:

Achieved an absolute lowest measured concentration of 100 aM.
Demonstrated significantly improved sensitivity compared to traditional end-point assays.
Reduced extrapolated limits of detection to 10 attomolar (less than 1300 copies/0.2 ml).
Decreased total incubation time from 5 hours to 35 minutes.

Conclusions:

The developed digital microarray with plasmonic gold nanorods and real-time tracking offers superior sensitivity and speed for miRNA detection.
This technology overcomes limitations of conventional assays, enabling detection of low-abundance biomarkers.
The enhanced detection capabilities hold promise for advancing miRNA-based diagnostics for various diseases.