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pSlugS158 Immunohistochemistry is Useful in Grading Meningioma
Chang Gok Woo1,2, Seung-Myoung Son1,2, Eung-Gook Kim3
1Department of Pathology, Chungbuk National University Hospital, Cheongju, Korea.
Abstract:
Snail family transcriptional repressor 2 (SNAI2, Slug) is a transcription factor that belong to the Slug/Snail superfamily. Site specific phosphorylation of slug (pSlugS158) is detected during the M phase, and thus, this phosphorylated protein is considered a novel marker for detecting mitotic figures. Herein, we investigated whether the detection of mitosis using pSlugS158 expression can be used in the histological grading of meningioma. We performed immunohistochemistry for pSlugS158 and PHH3 in tissue samples of 61 patients with meningioma and examined the association between mitotic counts using pSlugS158 and recurrence-free survival (RFS). The nuclear expression of pSlugS158 was observed in the cell with mitotic figures. Tumor grading based on pSlugS158 was significantly associated with the RFS (p < .001). It can be concluded that pSlugS158 is a useful and practical marker to detect mitosis and seems to be reliable for the counting of mitoses in histological grading of meningioma.
Insights
pSlugS158, a phosphorylated form of snail family transcriptional repressor 2 (SNAI2, Slug), effectively detects mitotic figures. This novel marker aids in meningioma histological grading and predicts recurrence-free survival.
Area of Science:
- Oncology
- Molecular Biology
- Pathology
Background:
- Snail family transcriptional repressor 2 (SNAI2, Slug) is a key transcription factor.
- Site-specific phosphorylation of Slug (pSlugS158) occurs during M phase, identifying it as a potential marker for mitotic figures.
Purpose of the Study:
- To evaluate pSlugS158 as a novel marker for detecting mitosis in meningioma.
- To assess the utility of pSlugS158 in meningioma histological grading and its association with recurrence-free survival (RFS).
Main Methods:
- Immunohistochemistry was performed on 61 meningioma tissue samples using pSlugS158 and PHH3 antibodies.
- Mitotic counts using pSlugS158 were analyzed for association with RFS.
Main Results:
- Nuclear expression of pSlugS158 was observed in cells with mitotic figures.
- Tumor grading based on pSlugS158 demonstrated a significant association with RFS (p < .001).
Conclusions:
- pSlugS158 is a practical and reliable marker for detecting mitosis in meningioma.
- pSlugS158 facilitates accurate histological grading of meningioma and aids in predicting patient outcomes.
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